TLR2 antibody (pAb)

Aliases: TIL4, CD282
Catalog No: 40981 Format: 100 µg $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
86 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This TLR2 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 180-196, 353-370, and 473-489 of human TLR2.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
TLR2 antibody (pAb) tested by Western blot.

TLR2 pAb tested by Western blot.
TBK1 detection by Western blot. The analysis was performed using TLR2 pAb at a 2 µg/ml dilution and 20 µg Ramos nuclear extract.

Background

TLR2Toll-Like receptor 2 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR2 expression is elevated after exposure to both Gram-positive and Gram-negative bacteria. TLR2 forms heterodimers with TLR1, TLR6, and possibly TLR10.

Positive Control

Ramos cell extracts can be used as a positive control for TLR2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting