BLM antibody (pAb)

Aliases: BS, RECQ2, RECQL2, RECQL3
Catalog No: 61055 Format: 100 µl $435 Buy
Catalog No: 61056 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
165 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This BLM antibody was raised against a peptide within the N-terminal region of human BLM.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
BLM antibody (pAb) tested by Western blot.

Western blot of BLM antibody.
Nuclear extract of HeLa cells (30 µg per lane) probed with BLM antibody (pAb) at a dilution of 1:500.

Background

BLM (Bloom Syndrome protein, RECQ2, RECQL3) is a DNA helicase similar to E. coli RecQ protein that is mutated in patients with Bloom Syndrome, an autosomal recessive disorder characterized by growth deficiency, sun sensitive skin, cancer predisposition and chromosomal instability. The five human RecQ helicases play critical roles in DNA recombination, replication and repair. BLM helps to resolve aberrant chromosome structures generated during DNA replication. It exists in a complex with topoisomerase IIIα, RMI1/BLAP75 and RMI2 and it is thought that BLM complexes are recruited to resolve Holliday junction intermediates. BLM is heavily phosphorylated, especially during mitosis. BLM has a role during meiosis as well, as BLM males are sterile and BLM females have reduced fertility.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for BLM antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting