FancD2 antibody (pAb)

Aliases: FA4, FAD, FACD, FAD2, FA-D2, FANCD
Catalog No: 61057 Format: 100 µl $435 Buy
Catalog No: 61058 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button

Antibody Type:
Affinity Purified
Molecular Weight:
150 kDa


Western Blot Validated Immunoprecipitation Validated

Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:5,000 dilution


This FancD2 antibody was raised against a peptide within the internal region of human FancD2.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


FancD2 antibody (pAb) tested by immunoprecipitation.

FancD2 antibody (pAb) tested by Immunoprecipitation.
10 µl of FancD2 antibody was used to immunoprecipitate FancD2 from 400 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.

FancD2 antibody (pAb) tested by Western blot.

Western blot of FancD2 antibody.
Lane 1: HeLa whole-cell extract (40 µg). Lane 2: Nuclear extract (40 µg) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.


FancD2 (Fanconi anemia complementation group D2) is a protein encoded by a gene mutated in Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. FancD2 is monoubiquitinated in response to DNA damage and is required for maintenance of chromosomal stability and the repair of DNA double-strand breaks. FancD2 also likely plays a role in activating the S-phase and G2/M DNA damage checkpoints. It promotes the loading of BRCA2 and FANCD1 onto damaged chromatin and may also be involved in B-cell immunoglobulin isotype switching.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for FancD2 antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting