ZEB1 antibody (pAb)

RRID: AB_2793514
Aliases: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1
Catalog No: 61119 Format: 100 µl $435 Buy
Catalog No: 61120 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
200 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This ZEB1 antibody was raised against a peptide derived from the N-terminal region of human ZEB1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
ZEB1 antibody (pAb) tested by immunoprecipitation.

ZEB1 antibody (pAb) tested by Immunoprecipitation.
10 µl of ZEB1 antibody was used to immunoprecipitate ZEB1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ZEB1 antibody at a dilution of 1:1,000.

ZEB1 antibody (pAb) tested by Western blot.

ZEB1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 μg) probed with ZEB1 antibody (1:1,000).

Background

ZEB1 (Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein Negative regulator of IL2, NIL-2-A, Transcription factor 8, TCF-8) is a POU domain DNA-binding transcriptional regulator that inhibits the expression of E-cadherin and other epithelial genes central to adhesion and epithelial polarity. ZEB1 induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 positively regulates neuronal differentiation and it represses RCOR1 transcription activation during neurogenesis. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3).

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ZEB1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting