Goosecoid antibody (pAb)

Aliases: GSC, SAMS
Catalog No: 61121 Format: 100 µl $435 Buy
Catalog No: 61122 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
40 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This Goosecoid antibody was raised against a peptide in the N-terminal region of human homeobox protein Goosecoid.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Goosecoid antibody (pAb) tested by Western blot.

Goosecoid antibody (pAb) tested by Western blot.
Detection of Goosecoid by Western blot analysis. Lane 1: Nuclear extract of HT-29 cells (30 µg). Lane 2: Nuclear extract of SW48 cells (30 µg).
Both were probed with Goosecoid antibody (pAb) at a 1:500 dilution.

Background

Goosecoid (Gsc) is a homeodomain transcription factor important for vertebrate development and organogenesis. First identified in Xenopus, it is expressed in the area of the Spemann organizer, a region within the embryo that control development and differentiation. Goosecoid regulates cell migration from the Spemann organizer and is overexpressed in a majority of human breast tumors. It is also involved in craniofacial and ribcage development.

Positive Control

Active Motif's SW48 nuclear extract (Catalog No. 36061) can be used as a positive control for Goosecoid antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting