STAT5B antibody (pAb)

Aliases: STAT5
Catalog No: 61137 Format: 100 µg $435 Buy
Catalog No: 61138 Format: 10 µg $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
90 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µg per IP
WB: 0.5 - 2 µg/ml dilution

Immunogen

This STAT5B antibody was raised against a peptide in the C-terminal region of human STAT5B.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
STAT5B antibody (pAb) tested by immunoprecipitation.

STAT5B antibody (pAb) tested by Immunoprecipitation.
10 µg of STAT5B antibody was used to immunoprecipitate STAT5B from 400 µg of K562 cytosolic cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the STAT5B antibody at a dilution of 1 µg/ml.

STAT5B antibody (pAb) tested by Western blot.

STAT5B antibody (pAb) tested by Western blot.
STAT5B antibody detection by Western blot. The analysis was performed using K-562 cytosolic-cell extract (25 µg) and STAT5B antibody (pAb) at a 1 µg/ml dilution.

Background

STAT5A and STAT5B are encoded by separate genes but are 90% identical. They mediate gene expression in response to IL-2, IL-3, IL-7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. The STAT5A/B signaling pathway is involved in the transition of organ-confined prostate cancer to hormone-refractory disease. This implies that the active JAK2-STAT5A/B signaling pathway potentially provides the means for pharmacological intervention of clinical prostate cancer progression.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting