DP1 antibody (pAb)

Aliases: TFDP1, Dp-1, DRTF1
Catalog No: 61145 Format: 100 µl $435 Buy
Catalog No: 61146 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This DP1antibody was raised against a recombinant protein corresponding to amino acids 301-410 of human DP1.

Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
DP1 antibody (pAb) tested by Western blot.

DP1 antibody (pAb) tested by Western blot.
Raji Nuclear cell extract (20 µg) probed with DP1 antibody at a dilution of 1:1,000.

Background

DP1 (DRTF1 polypeptide 1, TFDP1) is a transcriptional co-activator and an important regulator of cell cycle progression. It is a member of a protein family first identified through their binding to E2F DNA binding sites. DP1 forms heterodimers with E2F family members, activating transcription of E2F-responsive genes. DP1 is also absolutely required for extraembryonic development and embryonic survival.

Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for DP1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting