PBX2 antibody (mAb)

Clone: 2.1
Aliases: G17, HOX12, PBX2MHC
Catalog No: 61155 Format: 100 µg $435 Buy
Catalog No: 61156 Format: 10 µg $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
51 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes


Published Applications:
IHC (FFPE)
WB
See references for more information. Individual optimization may be required.

Also available as an AbFlex® engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91227.

Immunogen

This PBX2 antibody was raised against a recombinant protein corresponding to amino acids 308 - 430 of mouse PBX2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

PBX proteins are a family of TALE-class transcription factors that subset of Hox proteins, enhancing their DNA binding affinities and specificities. PBX proteins also heterodimerize with the Meis/Prep subfamily of to form trimeric complexes with Hox proteins at specific DNA sites. PBX2 is the predominant high-molecular-weight isoform PBX protein detectable by immunoblotting and is widely expressed during embryonic development, particularly in neural and epithelial tissues.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting