Histone H3.X/Y antibody (mAb)

RRID: AB_2793533
Clone: 8H6-2111
Catalog No: 61161 Format: 100 µg $445 Buy
Catalog No: 61162 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
17 kDa
Reactivity:
Human

Applications

CUT&RUN Validated ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

CUT&RUN

The clone 8H6-2111 has been published, but not tested by Active Motif, for ICC/IF and Western blot applications.

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

Buffer

Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80% conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3.X/Y antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting