LIN28A antibody (pAb)

Aliases: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
Catalog No: 61191 Format: 100 µl $435 Buy
Catalog No: 61192 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
30 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:200 dilution
WB: 1:500 - 1:1,000 dilution

Immunogen

This LIN28A antibody was raised against a peptide derived from the C-terminal region of human LIN28A.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
LIN28A antibody (pAb) tested by immunofluorescence.

Immunocytochemical analysis of LIN28A staining of mouse embryonic stem cells.
Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with LIN28A antibody (Catalog No. 61191, red) at 1:200 dilution overnight at 4°C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Nuclei were stained with DAPI (blue). Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that LIN28A antibody specifically stains mESC colonies and does not stain MEFs. Absence of LIN28A staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 μm.

LIN28A antibody (pAb) tested by Western blot.

LIN28A antibody (pAb) tested by Western blot.
Cytosolic extract (40 µg) of mouse ES cells probed with LIN28A antibody at a dilution of 1:500.

Background

LIN28 is an evolutionarily conserved RNA-binding protein that serves as a marker of undifferentiated human embryonic stem cells. LIN28 can facilitate the reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with LIN28A antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting