HSP72 antibody (mAb)

Clone: 3G7
Aliases: HSPA1A, HSPA1, HSP70I, HSP70-1, HSP70-1A, HEL-S-103
Catalog No: 61209 Format: 100 µg $435 Buy
Catalog No: 61210 Format: 10 µg $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
70 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This HSP72 antibody was raised against full-length recombinant human HSP72 protein.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

HSP72 (Heat Shock Protein 72) is a member of the DnaK/HSP70 family of protein chaperones involved in protein homeostasis. HSP72 is expressed at low constitutive levels and is induced upon exposure to environmental stress. HSP72 released into the blood can serve as an alert for the immune system. Increased levels of HSP72 can repress expression of inflammatory response genes by inhibiting NF-κB.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HSP72 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting