PHB / Prohibitin antibody (pAb)

Aliases: PHB1, HEL-215, HEL-S-54e
Catalog No: 61217 Format: 100 µl $435 Buy
Catalog No: 61218 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
30 kDa


Western Blot Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.


This PHB / prohibitin antibody was raised against a peptide within the N-terminal region of human PHB.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


PHB / Prohibitin antibody (pAb) tested by Western blot.

PHB / Prohibitin antibody (pAb) tested by Western blot.
Whole-cell extract of MCF-7 cells (25 µg) probed with PHB antibody (pAb) at a dilution of 1:500.


PHB / Prohibitin is a key cell cycle regulatory protein, inhibiting cell division by inactivating E2F family proteins, and has potent anti-proliferative activity. PHB / Prohibitin is also involved in repression of transcription through androgen receptor.

Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for PHB / Prohibitin antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting