5-Carboxylcytosine (5-caC) antibody (pAb)

RRID: AB_2793557
Catalog No: 61225 Format: 100 µl $460 Add to Cart
Catalog No: 61226 Format: 10 µl $115 Add to Cart
Catalog No: 61925 Format: 50 µl $260 Add to Cart

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Reactivity:
Not Species Specific

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
DB: 1:5,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF
IHC(P)
WB
DB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

5-Carboxylcytosine (5-caC) antibody (pAb) - 100 µg (Cat. No. 61225)

 
 
 

 
5-Carboxylcytosine (5-caC) antibody (pAb) tested by dot blot analysis.

Dot blot of 5-Carboxylcytosine pAb.
Dot blot analysis was used to confirm the specificity of 5-Carboxylcytosine antibody for 5-Carboxylcytidine. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 1:2,000. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-methylcytidine. Lane 5: oligo containing unmodified cytidine.

Background

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting