Cart
Products
EZH2 phospho Thr345 antibody (pAb)
| RRID: AB_2793564 |
| Aliases: KMT6, Enx-1, Enx1h, mKIAA4065 |
| Catalog No: 61241 | Format: 100 µl | Discontinued | |
| Catalog No: 61242 | Format: 10 µl | Discontinued | |
Applications
Application Notes
This product has been discontinued:
Please see our alternative antibodies for EZH2 Catalog No. 39901 and Catalog No. 39933
Published Applications:
WB
See references for more information. Individual optimization may be required.
Immunogen
This EZH2 phospho Thr345 antibody was raised against a peptide containing phosphothreonine 345 of mouse EZH2 (enhancer of zeste homolog 2 (Drosophila))
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
EZH2 phospho Thr345 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of EZH2 phospho Thr345 antibody. Modified and unmodified peptide were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Decreasing amounts of peptide were spotted in each row.
Lane 1: Peptide phosphorylated at threonine 345.
Lane 2: Unmodified EZH2 peptide.
Background
EZH2 – (Enhancer of Zeste homolog 2, also designated Enx1) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. Threonine 345 of mouse Ezh2 is phosphorylated in a cell cycle-dependent manner and may play a role in the interaction of Ezh2 with HOTAIR and the 59-RepA repeats of Xist. (see reference)
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
