PELP1 antibody (pAb)

Aliases: Proline, Glutamate And Leucine Rich Protein 1, HMX3, MNAR, P160
Catalog No: 61263 Format: 100 µl $435 Buy
Catalog No: 61264 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
150 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This PELP1 antibody was raised against a peptide within the internal region of human PELP1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
PELP1 antibody (pAb) tested by Western blot.

PELP1 antibody (pAb) tested by Western blot
Whole cell extract of MCF-7 cells (30 µg) probed with PELP1 antibody (1:500).

Background

The PELP1 gene (proline, glutamic acid and leucine rich protein 1, Modulator of non-genomic activity of estrogen receptor, HMX3, MNAR, P160) encodes a protein that is a coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. PELP1 interacts with KDM1 (LSD1) and may act as reader of histone methyl modifications for ER mediated gene activation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting