PDX1 antibody (pAb)

RRID: AB_2793583
Aliases: Pancreatic And Duodenal Homeobox 1, Insulin Promoter Factor 1, Homeodomain Transcription Factor, Somatostatin-Transactivating Factor 1
Catalog No: 61289 Format: 100 µl $445 Buy
Catalog No: 61290 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
42 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Immunogen

This PDX1 antibody was raised against a peptide within the N-terminal region of human PDX1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

PDX1 pAb tested by Western blot.
PDX1 detection by Western blot. The analysis was performed using 30 µg Caco-2 nuclear cell extract and PDX1 at a 1:500 dilution.

Background

PDX1 (pancreatic and duodenal homeobox 1, GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1) activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Positive Control

Active Motif's Caco-2 nuclear extract (Catalog No. 36035) can be used as a positive control for PDX1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting