JunD antibody (pAb)

Aliases: AP-1
Catalog No: 61403 Format: 100 µl $445 Buy
Catalog No: 61404 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
40 and 45 kDa
Reactivity:
Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
ChIP-Seq: 2 - 5 µl per ChIP
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Immunogen

This antibody was raised against a peptide within the N-terminal region of human JunD.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
JunD antibody (pAb) tested by ChIP-Seq.

JunD antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human endometrial stromal cell chromatin and 4 µg of JunD antibody. ChIP DNA was sequenced on the Illumina HiSeq and 8.6 million sequence tags were mapped to identify JunD binding sites on chromosome 16. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

JunD antibody (pAb) tested by immunoprecipitation.

JunD antibody (pAb) tested by Immunoprecipitation.
10 µl of JunD antibody was used to immunoprecipitate JunD from 250 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JunD antibody at a dilution of 1:1,000.

JunD antibody (pAb) tested by Western blot.

JunD antibody (pAb) tested by Western blot.
K-562 nuclear extract (20 µg) probed with JunD antibody at a dilution of 1:1,000.

Background

JunD (Jun D proto-oncogene) is a member of the JUN family and forms a component of the AP-1 transcription factor complex. JunD binds DNA as a dimer. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for JunD antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with JunD antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting