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KMT2E (MLL5) antibody (pAb)

Aliases: NKp44L, HDCMC04P
Catalog No: 61447 Format: 100 µl $425 Buy Now
Catalog No: 61448 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:85 kDa
Reactivity:Human, Mouse


Western Blot Validated

for KMT2E (MLL5) antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

for KMT2E (MLL5) antibody (pAb)Immunogen

This antibody was raised against a peptide within human KMT2E (MLL5).

for KMT2E (MLL5) antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for KMT2E (MLL5) antibody (pAb)References

KMT2E (MLL5) antibody (pAb) tested by Western blot.

KMT2E (MLL5) (pAb) tested by Western blot.
Detection of MLL5 / KMT2E by Western blot analysis. Nuclear extract of mouse testis (40 µg) was probed with KMT2E (MLL5) (pAb) at a 1:2,000 dilution.

for KMT2E (MLL5) antibody (pAb)Background

KMT2E (MLL5) (myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila / lysine N-methyltransferase 2E)) is a histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases.

for KMT2E (MLL5) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for KMT2E (MLL5) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

KMT2E (MLL5) antibody (pAb)

Data Thumbnails

KMT2E (MLL5) antibody (pAb) tested by Western blot.

Western blot of KMT2E (MLL5) pAb.
(Click image to enlarge and see details.)