Histone H3K37ac antibody (pAb)

Catalog No: 61587 Format: 100 µl $435 Buy
Catalog No: 61588 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
17 kDa
Human, Other (Wide Range)


Western Blot Validated Immunofluorescence Validated Dot Blot Validated

Application Notes

Validated Applications:
WB: 1:2,000 - 1:10,000 dilution
IF: 1:500 dilution

Published Applications:

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a synthetic peptide containing acetyl-lysine 37 of human Histone H3.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.


Histone H3K37ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys37 antibody (pAb) tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys37 antibody at a 1:2,500 dilution.
Lane 1: no treatment.
Lane 2: cells treated with sodium butyrate.

Histone H3K37ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys37 (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys37 (pAb) for acetyl Lys37 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a 1:2,500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: H3 acetyl-Lys37 peptide. Lane 2: unmodified H3 peptide. Lane 3: H3 acetyl-Lys36 peptide. Lane 4: H3 acetyl-Lys9 peptide. Lane 5: H3 acetyl-Lys14 peptide. Lane 6: H3 acetyl-Lys18 peptide. Lane 7: H3 acetyl-Lys23 peptide. Lane 8: H3 acetyl-Lys27 peptide. Lane 9: H4 acetyl-Lys5 peptide. Lane 10: H4 acetyl-Lys8 peptide. Lane 11: H4 acetyl-Lys12 peptide. Lane 12: H4 acetyl-Lys16 peptide.

Histone H3K37ac antibody (pAb) tested by immunofluorescence.

Detection of H3K37ac by immunofluorescence
U2OS cells were stained with H3K37ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H3K37ac antibody staining. Right panel: merge.


Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K37ac antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting