TNF antibody (mAb)

RRID: AB_2793696
Aliases: DIF, TNFA, TNFSF2, TNF-alpha
Catalog No: 61601 Format: 100 µg $460 Buy
Catalog No: 61602 Format: 10 µg $115 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
17 kDa
Reactivity:
Human

Applications

Enzyme-linked Immunosorbent Assay Validated

Application Notes

Applications Validated by Active Motif:
ELISA: Individual optimization may be required.

Immunogen

Human TNF.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

TNF antibody (mAb) tested by ELISA.
Recombinant TNF protein or a negative control protein was conjugated to Luminex® microspheres and then incubated in the same well with a serial dilution of TNF antibody (mAb).  Biotinylated anti-mouse IgG followed by Streptavidin-PE was used to detect the bound TNF antibody . Mean Fluorescence Intensity (MFI) for each protein was measured in a Luminex® 200™.

Background

TNF (Tumor Necrosis Factor) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting