Histone H2AQ104me1 antibody (pAb)

Catalog No: 61605 Format: 100 µl $435 Buy
Catalog No: 61606 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
14 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide containing human histone H2AQ104me1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Histone H2AQ104me1 antibody (pAb) tested by Western blot.

Histone H2AQ104me1 antibody (pAb) tested by Western blot.
Histone H2AQ104me1 detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear cell extract and Histone H2AQ104me1 antibody at a 1:500 dilution.

Histone H2AQ104me1 antibody (pAb) tested by dot blot analysis.

Histone H2AQ104me1 (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2AQ104me1 pAb for monomethyl-glu104 of histone H2A. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000.
    Lane 1: Unmodified glutamine 104 of H2A peptide.
    Lane 2: Monomethylated glutamine 104 of H2A peptide.

Background

Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (Histone H2A, Histone H2B, Histone H3, and Histone H4). Methylation on glutamine 104 has been implicated in RNA Pol-I transcriptional regulation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting