NR0B1 antibody (pAb)

Aliases: AHC, AHX, DSS, GTD, HHG, AHCH, DAX1, DAX-1, NROB1, SRXY2
Catalog No: 61661 Format: 100 µl $435 Buy
Catalog No: 61662 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
60 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This antibody was raised against recombinant human NR0B1 protein.

Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

References

 
NR0B1 antibody (pAb) tested by Western blot.

NR0B1 antibody (pAb) tested by Western blot.
Detection of NR0B1 by Western blot. Jurkat whole cell extract (20 µg) probed with NR0B1 antibody at a 1:500 dilution.

Background

NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1) is an orphan nuclear receptor and a component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. NR0B1 inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. It may also have a role in embryonic development and in the maintenance of embryonic stem cell pluripotency as it up-regulates Oct-4 expression ES cells. Defects in NR0B1 are a cause of dosage-sensitive sex reversal (DSSR) and X-linked adrenal hypoplasia congenital (XL-AHC). DSSR results from a duplication of the region of the X-chromosome containing NR0B1, resulting in males with two copies of NR0B1, which inhibits normal testis formation, and thus XY individuals develop as females. XL-AHC is a developmental disorder of the adrenal gland, characterized by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting