Histone H3.3S31ph antibody (mAb)

Clone: 1A8G10
Catalog No: 61671 Format: 100 µg $435 Buy
Catalog No: 61672 Format: 10 µg $105 Buy

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Antibody Type:
Protein G Chromatography
Molecular Weight:
17 kDa
Human, Other (Wide Range)


Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
ICC/IF: 5 µg/ml dilution
WB: 0.5 - 2 µl/ml dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a synthetic peptide including phospho-serine 31 of human histone H3.3.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.


Histone H3.3S31ph antibody (mAb) tested by immunofluorescence.

Histone H3.3S31ph antibody (mAb) (Clone 1A8G10) tested by immunofluorescence.
Left: HeLa cell stained with H3.3S31ph antibody (mAb). Right: Hoechst.

Histone H3.3S31ph antibody (mAb) tested by Western blot.

Histone H3.3S31ph antibody (mAb) (Clone 1A8G10) tested by Western blot.
Detection of Histone H3.3S31ph antibody by Western blot. The analysis was performed using 20 µg of untreated (lane 1) or cocemid treated (lane 2) HeLa nuclear extract with Histone H3.3S31ph antibody at a 1 µg/ml dilution.


Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3.3S31ph antibody (mAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting