C17orf96 phospho Ser15 antibody (pAb)

Aliases: E13, AA409164, AI413509, AW539173, esPRC2p48
Catalog No: 61745 Format: 100 µl $435 Buy
Catalog No: 61746 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Mouse

Applications

Dot Blot Validated

Application Notes


Validated Applications:
DB: 1:1,000 dilution

Published Applications: see references

Immunogen

This antibody was raised against a synthetic peptide containing phospho-serine 15 of human C17orf96.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
C17orf96 phospho Ser15 antibody (pAb) tested by dot blot analysis.

C17orf96 phospho Ser15 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of C17orf96 phospho Ser15 antibody for phosphorylation of serine 15 of C17orf96. Peptides corresponding to the immunogen and related sequences derived were spotted onto PVDF and probed with the antibody at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho serine 15 peptide.
    Lane 2: Unmodified serine 15 peptide.
    Lane 3: Unrelated phospho serine peptide.

Background

C17orf96 has been shown to be highly expressed in mammalian ES cells and has been implicated as an interacting protein of the PRC2 complex (polycomb repressive complex 2). It may function as a CpG island binding protein and thus a potential regulator of gene transcription.

Phosphorylation of C17orf96 may be involved in regulation of the interaction of C17orf96 with PRC2.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting