CDX1 antibody (mAb)

Catalog No: 61791 Format: 100 µg $435 Buy
Catalog No: 61792 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated

Application Notes


Published Applications:
ChIP
WB
IHC
IF
Chan et. al., Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1936-41 (Western Blot, IHC, ChIP)
Jones et al. 2015. Proc Natl Acad Sci U S A. 112(13):E1550-8. (ChIP)
Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. (IHC, IF)

Immunogen

This antibody was raised against an N-terminal peptide (a.a. 30-47) of human CDX1 (NP_001795).

Buffer

PBS containing 30% glycerol and 0.035% sodium azide.

References

Background

The CDX family members (CDX1, CDX2, and CDX4) are Caudal-related transcription factors implicated in anterior-posterior patterning during embryonic development, regulation of cardiac differentiation in stem cells and down-regulation of genes associated with neuronal growth and vascular endothelial growth. The caudal-type homeobox 1 (CDX1) transcription factor controls enterocyte differentiation in the colon and is also central to the capacity of a colorectal cancer (CRC) cell line to differentiate. CDX1 is a negative marker of CRC stem cells. The expression of CDX1 is itself regulated by methylation at the CDX1 promoter region, and its expression is conversely correlated with expression of the polycomb complex protein BMI-1, which is involved in maintenance of quiescent stem cells and is expressed in cancer stem cells.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CDX1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use CDX1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CDX1 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting