Histone H2A.J antibody (pAb)

Catalog No: 61793 Format: 100 µl $435 Buy
Catalog No: 61794 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Human, Mouse


Western Blot Validated Dot Blot Validated

Application Notes

Validated Applications:
Western Blot: 1:500 - 1:2500
Dot Blot: 1:1000

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


Histone H2A.J antibody (pAb) tested by Western blot.

Western blot of Histone H2A.J pAb.
20 µg Nuclear Extract of HeLa cells (Lane 1) or 40 µg mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3).

Histone H2A.J antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H2A.J pAb.
Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.


Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting