Bcl7A antibody (pAb)

Aliases: B-cell CLL/lymphoma 7 protein family member A, BCL Tumor Suppressor 7A, BAF Chromatin Remodeling Complex Subunit BCL7A, BAF Complex Component,
Catalog No: 65695 Format: 100 µl $460 Buy
Catalog No: 65696 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
45 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 dilution
IP: 5 µl per IP

Immunogen

This antibody was raised against a peptide within human BCL7A.

Buffer

Purified IgG in 70 mM Tris, 105 mM NaCl, 0.07 mM EDTA, 31 mM glycine, 0.035% sodium azide, 30% glycerol, and 0.5mg/mL BSA.

 

Bcl7 antibody (pAb) tested by Immunoprecipitation
5 µl of Bcl7A antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Bcl7A from 500 µg of HEK293 whole cell extract. The immunoprecipitated protein was detected by Western blotting using a the same Bcl7A antibody at a dilution of 1:1000 (crossreaction between heavy and light chains of the antibody is observed and is common).

Bcl7 antibody (pAb) tested by Western blot
20 µg of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at a dilution of 1:500. The calculated M.W. of Bcl7A is 23 kDa, whereas it's observed M.W. on SDS-PAGE is 45 kDa.

Background

Bcl7A (B-cell CLL/lymphoma 7 protein family member A) is a gene which was identified in translocation in Burkitt Lymphoma cells, and has been associated with chromosomal aberrations leading to B-cell non-Hodgkin lymphoma, and is expressed in the majority of precursor and mature B cell lymphomas.The three known BCL7 proteins (BCL7A, BCL7B, and BCL7C) share sequence homology and may play a role in chromatin remodeling.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting