Recombinant RNA Pol II - CTD protein

Catalog No: 81036 Format: 10 µg $405 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant RNA Pol II - CTD protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant RNA Pol II - CTD protein corresponding to amino acids 1489-1970 of RNA polymerase II subunit A (accession number NP_000928.1) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. Recombinant RNA Pol II - CTD protein consists of 52 heptapeptide repeats of the consensus sequence Y1-S2-P3-T4-S5-P6-S7. The molecular weight of Pol II - CTD protein is 50.7 kDa.

 
Recombinant RNA Pol II - CTD protein gel

Recombinant RNA Pol II - CTD protein gel
8% SDS-PAGE gel stained with Coomassie blue.
Predicted M.W.: 50.7 kDa
Observed M.W.: 75 kDa
Purity: > 70%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.