Recombinant Histone H1T

Catalog No: 81127 Format: 100 µg $150 Buy
Catalog No: 81827 Format: 1 mg $850 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.

Background

Histone H1T, also called as HIST1H1T (Histone Cluster 1 H1 Family Member T) or Testicular H1 Histone, is a replacement subtype, and replication-independent histone, which is a member of the histone H1 family.

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker- histone H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone H1T forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination.

Application Notes

Recombinant Histone H1T is suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details

Full length Histone H1T (accession number AAA35944.1) was expressed in E. coli cells with no tag. The molecular weight of Histone H1T is 22 kDa.

 
Recombinant Histone H1T protein gel

Recombinant Histone H1T protein gel
12.5% SDS-PAGE Coomassie staining
MW: 22 kDa
Purity: >65%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.