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Recombinant TMEM173 (STING) (149-379) protein
| Catalog No: 81182 | Format: 50 µg | $450 | Buy |
| Catalog No: 81882 | Format: 1 mg | $3,000 | Buy |
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Expressed In: E. coli Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
STING (Stimulator Of Interferon Genes, also known as TMEM173, HMITA, NET23, ERIS, MPYS, MITA, SAVI) is a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. STING is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. It has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. It acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant TMEM173 (STING) (149-379) corresponding to amino acids 149-379 of human STING (accession number NP_938023.1) was expressed in E. coli cells and contains a C-terminal 6×His tag with a molecular weight of 27.3 kDa.
Recombinant TMEM173 (STING) (149-379) gel
12.5% SDS-PAGE Coomassie staining
M.W.: 27.3 kDa
Purity: ≥ 65%
cGAMP Kd test for Recombinant TMEM173 (STING) (149-379)
Serial dilutions of cGAMP was incubated with Recombinant TMEM173 in reaction buffer including 20 mM Tris-HCl pH 7.5 and 150 mM NaCl at room temperature. Dye solution was added and the reaction was read on ViiA7qPCR system (ex/em=470/558, 25°C to 95°C ).
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
