Recombinant YEATS4 (GAS41) protein

Catalog No: 81193 Format: 100 µg $485 Buy
Catalog No: 81793 Format: 1 mg $3,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

YEATS4 also known as GAS41, is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is found in the nucleoli and has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating YEATS4 protein might also represent a transcription factor.

Application Notes

Recombinant YEATS4 / GAS41 protein is suitable for use in binding assay, inhibitor screening, and selectivity profiling.

Protein Details

Recombinant YEATS4 / GAS41 protein as the full length protein (accession number NP_006521.1) was expressed in E. coli cells with an N-terminal 6xHis Tag and a C-terminal FLAG-Tag. The molecular weight of the protein is 31 kDa.

 

Recombinant YEATS4 / GAS41 protein
12.5% SDS-PAGE Coomassie staining
MW: 31 kDa
Purity: > 81%

Recombinant YEATS4 / GAS41 protein activity assay
3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 µl reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Recombinant YEATS4 / GAS41 protein activity assay
3 µM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 µl reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 µl FLAG antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.