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Antibodies

AbFlex® EHMT2 (G9a) antibody (rAb)

Catalog No: 91229 Format: 100 µg $375 Buy Now
Catalog No: 91230 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:160 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex EHMT2 (G9a) antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/mL

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AbFlex EHMT2 (G9a) antibody (rAb)Immunogen

This antibody was raised against full length human recombinant EHMT2 (G9a) protein.

for AbFlex EHMT2 (G9a) antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex EHMT2 (G9a) antibody (rAb)References

 
AbFlex<sup>®</sup> EHMT2 (G9a) antibody (rAb) tested by Western blot.

AbFlex® EHMT2 (G9a) antibody (rAb) tested by Western blot.
20 µg of HEK293 cell nuclear extract was run on SDS-PAGE and probed with AbFlex EHMT2 (G9a) antibody at 2 µg/ml.

for AbFlex EHMT2 (G9a) antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex EHMT1 (G9a) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

EHMT2 (G9a) is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2 (G9a) also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. EHMT2 (G9a) also weakly methylates Lys27 of histone H3 (H3K27me). EHMT2 (G9a) is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. EHMT2 (G9a) may also methylate histone H1.

for AbFlex EHMT2 (G9a) antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex EHMT2 (G9a) antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® EHMT2 (G9a) antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> EHMT2 (G9a) antibody (rAb) tested by Western blot.

Western blot of AbFlex® EHMT2 (G9a) antibody (rAb).
(Click image to enlarge and see details.)