AbFlex® HA-Tag antibody (rAb)

RRID: AB_2793828
Catalog No: 91271 Format: 100 µg $350 Buy
Catalog No: 91272 Format: 10 µg $75 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Reactivity:
Human, Not Species Specific

Applications

Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
IP: 2 - 5 µg per IP

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39627.

Immunogen

This antibody was raised against a peptide containing multiple repeats of the HA tag epitope YPYDVPDYA.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 

AbFlex® HA-Tag antibody (rAb) tested by Immunoprecipitation.
Whole-cell lysate (400 µg) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 µg of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb Cat. No. 39627 at 4 µg/ml.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® HA-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting