New Products for Epigenetics Research

  • Low Cell ChIP-Seq Kit

    Obtain high quality histone and transcription factor ChIP-Seq data from as few as 1,000 cells using Active Motif's Low Cell ChIP-Seq Kit. Complete ChIP-Seq workflow includes reagents for chromatin preparation, immunoprecipitation and NGS library preparation that have been optimized for use on limited cell numbers. More >

  • Cell-Free DNA (cfDNA) Purification Kit

    Isolate cell-free DNA from human plasma and serum samples using our Cell-Free DNA Purification Kit. Our fast, magnetic bead-based protocol makes it easy to isolate and concentrate cell-free DNA from fresh or frozen samples. More >

  • Mod Spec Services

    Mod Spec is a mass spectrometry method that is used to measure the relative abundance of > 80 different histone modifications. Since total nuclear levels of histone PTMs may differ under varying conditions – disease vs normal, DMSO vs inhibitor, or WT vs KO, this technique can be very useful in verifying expected and unexpected differences in those levels. More >

  • ATAC-Seq Services

    ATAC-Seq provides genome-wide profiles of open and accessible regions of chromatin. This assay is based on transposase-mediated insertion of sequencing primers into those regions and it can be undertaken with as few as 100,000 cells. Learn More >

  • FFPE ChIP-Seq Services

    FFPE ChIP-Seq Services: Perform ChIP-Seq from clinical samples using limited amounts of formalin-fixed, paraffin-embedded (FFPE) tissue (blocks, slides, or sections). Learn More >

  • Interactome Profiling Service (RIME): Identify Protein Interactions by Mass Spectrometry

    Adding to our growing list of Epigenetic Services offerings, Active Motif now offers RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins), which allows for the identification of transcriptional co-factors and chromatin associated proteins. More >

  • AbFlex® Recombinant Antibodies (rAb)

    Active Motif's unique AbFlex® Recombinant Antibodies are generated from defined DNA sequences ensuring consistency and specificity across antibody lots. AbFlex antibodies contain three molecular tags for flexible, site-specific labeling and purification options. More >

  • Sortag-IT™ Antibody Labeling Kits

    The Sortag-IT™ Labeling Kits are designed for use in combination with Active Motif's AbFlex® Recombinant Antibodies. Easily attach a variety of labels including fluorophores, enzymes (AP, HRP), biotin, DNA, peptides or other labels directly to the antibody via the Sortase recognition sequence. More >

  • FFPE Bisulfite Conversion Kit

    The FFPE Bisulfite Conversion Kit is designed for the analysis of DNA methylation profiles in FFPE tissue sections. It offers the advantage of providing optimized reagents throughout the workflow to improve recovery of high quality DNA, minimize degradation and provide greater than 99% conversion efficiency. Learn More >

  • enChIP Kit to Confirm CRISPR/Cas9 Specificity

    The enChIP Kit uses the CRISPR/Cas9 system to direct a guide RNA (gRNA) and AM-tagged dCas9 protein to a specific genomic locus for immunoprecipitation. enChIP can be used to identify off-target binding effects. Learn More >

  • Quantify Global Changes in 5-hmC

    The Global 5-hmC Quantification Kit is an ELISA-based assay designed to screen for differences in global 5-hydroxymethylcytosine (5-hmC) levels across samples or treatment conditions. The included DNA standards provide quantitative results that are consistent with mass spectrometry. Learn More >

  • Specific Isolation of Nuclear and Cytoplasmic RNA

    The RNA Subcellular Isolation Kit makes it easier to study low abundance RNA transcripts and to demonstrate the role of lncRNA localization with RNA function by providing an efficient method to isolate nuclear or cytoplasmic RNA fractions without cross-contamination. Learn More >

  • Perform ChIP Without Protein-specific Antibodies

    The Tag-ChIP-IT® Kit makes it easy to perform ChIP with transcription factor sequence variants, mutations or truncations by eliminating the need for protein-specific antibodies. Simply clone your protein sequence with Active Motif's unique AM-tag, specifically designed for use in ChIP, transfect your cells and perform ChIP using an antibody directed against the AM-tag. Learn More >

  • Promoter Reporters Validated Using Pathway-specific Inductions

    The LightSwitch™ Validated Pathway Collections contain promoter reporter constructs that were experimentally validated to show significant activation or repression in response to pathway-specific induction conditions, so they are ideal for studying the key promoters that regulate a variety of different pathways, such as inflammation, hypoxia, DNA damage, heat shock, etc. Learn More >

  • Reduce the Effects of Technical Variation In Your ChIP

    Active Motif has developed a ChIP Normalization Strategy that can be used to normalize ChIP qPCR and ChIP-Seq data to reduce the effects of technical variation and sample processing bias. This normalization strategy can be applied across samples and antibodies. Learn More >

  • New Recombinant Nucleosomes for use in Enzymatic Assays and Chromatin Assembly

    Active Motif's Recombinant Nucleosomes offer a more biologically relevant choice of substrate when compared to histone proteins alone or synthetic peptides for use in drug discovery screening, analyzing enzyme kinetics or monitoring changes in histone modifications. Each recombinant nucleosome is comprised of octamers of unmodified core histone proteins (H2A, H2B, H3 and H4) bound by DNA. Biotin-tagged Nucleosomes are also available for pull-down or binding assays. Learn More >