Rad51 antibody (pAb)

for Rad51 antibody (pAb)Background

Rad51 (BRCC5, RecA) is known to be involved in the homologous recombination and repair of DNA. Rad51 can interact with the ssDNA-binding protein RPA and Rad52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Rad51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

for Rad51 antibody (pAb)Immunogen

This antibody was raised against full-length human Rad51 expressed in E. coli.

for Rad51 antibody (pAb)Application Notes

This Rad51 antibody has been validated for use in Western blotting (0.5 - 2 µg/ml) and immunofluorescence (1 - 5 µg/ml) and confirmed by siRNA knockdown of Rad51 mRNA in both. It has been reported to work in immunoprecipitation.

for Rad51 antibody (pAb)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide.

for Rad51 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad51 antibody.

for Rad51 antibody (pAb)References

This antibody was used in Bennett and Knight (2005) J Cell Biochem 96:1095.

for Rad51 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Rad51 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.