| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Protein A Chromatography |
|
| Appl.: | IF, IP, WB |
| Reactivity: | Human |
| Molecular Weight: | 37 kDa |
|
| Western Blot |
Chromatin IP |
Immunofluorescence |
Immunoprecipitation |
| 0.5 - 2.0 µg/ml |
Not tested |
1 - 5 mg/ml |
3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see “Application Notes” below for any recommendations. |
Background for Rad51 antibody (pAb):
Rad51 (
BRCC5,
RecA) is known to be involved in the homologous recombination and repair of DNA.
Rad51 can interact with the ssDNA-binding protein RPA and
Rad52, and it is thought to play roles in homologous pairing and strand transfer of DNA.
Rad51 is also found to interact with
BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.
Immunogen for Rad51 antibody (pAb):
This antibody was raised against full-length human Rad51 expressed in
E. coli.
Application Notes for Rad51 antibody (pAb):
This Rad51 antibody has been validated for use in Western blotting (0.5 - 2 µg/ml) and immunofluorescence (1 - 5 µg/ml) and confirmed by siRNA knockdown of Rad51 mRNA in both. It has been reported to work in immunoprecipitation.
Buffer:
PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide.
Positive Control:
Active Motif's
293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad51 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
References:
This antibody was used in Bennett and Knight (2005)
J Cell Biochem 96:1095.
Rad51 pAb tested by immunofluorescence.
HEK293 cells stained with Rad51 polyclonal antibody (green) and DAPI (blue).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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