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Cleavage Under Target and Tagmentation (CUT&Tag) is an alternative method to traditional ChIP assays that can reveal interactions between histone modifications and other proteins and the DNA they interacts with or to identify DNA binding sites for proteins of interest. The CUT&Tag assay requires a highly specific primary antibody to the protein target of interest that must specifically detect the protein in its native, unfixed form. Active Motif specializes in manufacturing high-quality antibodies to histones, histone modifications, chromatin proteins, and other factors, including a growing list of antibodies that we have experimentally validated in-house to work well in CUT&Tag assays.
Active Motif Antibodies Published for Use in CUT&Tag
The following applications have been published using these antibodies and is provided for reference. Active Motif may not have validated these antibodies for use in these applications.
CUT&Tag Publications
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The antibodies in the table below have been validated for use in our CUT&Tag-IT® Assay Kits. Click on the antibody name to see its complete information.
| Name | Isotype | Reactivity | Appl. | Format | Cat No. | Price | |
|---|---|---|---|---|---|---|---|
| AbFlex® CTCF antibody (rAb) | IgG | Human | ChIP-Seq, CUT&Tag, WB | 100 µg | 91285 | $385 | Buy |
| 10 µg | 91286 | $85 | Buy | ||||
| CTCF antibody (pAb) | IgG | Human | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, EMSA, ICC, IF, IHC, WB | 100 µl | 61311 | $510 | Buy |
| 10 µl | 61312 | $125 | Buy | ||||
| 50 µl | 61932 | $295 | Buy | ||||
| Histone H2A.Z antibody (pAb) | Serum | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, IF, WB | 100 µl | 39113 | $510 | Buy |
| 50 µl | 39013 | $295 | Buy | ||||
| 10 µl | 39114 | $125 | Buy | ||||
| Histone H3K4me1 antibody (mAb) | IgG2b | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, WB | 100 µg | 39635 | $510 | Buy |
| AbFlex® Histone H3K4me1 antibody (rAb) | IgG | Human, Wide Range Predicted | ChIP-Seq, CUT&Tag, WB | 100 µg | 91415 | $385 | Buy |
| 10 µg | 91416 | $85 | Buy | ||||
| Histone H3K4me2 antibody (pAb) | Serum | Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, WB | 100 µl | 39141 | $510 | Buy |
| 50 µl | 39042 | $295 | Buy | ||||
| 10 µl | 39142 | $125 | Buy | ||||
| AbFlex® Histone H3K4me3 antibody (rAb) | IgG2a | Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, WB | 100 µg | 91263 | $385 | Buy |
| 10 µg | 91264 | $85 | Buy | ||||
| Histone H3K4me3 antibody (pAb) | Serum | Budding Yeast, Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, IHC, WB | 100 µl | 39159 | $510 | Buy |
| 50 µl | 39060 | $295 | Buy | ||||
| 10 µl | 39160 | $125 | Buy | ||||
| Histone H3K4me3 antibody (pAb) | IgG | Budding Yeast, Human, Wide Range Predicted | ChIP, ChIP-Seq, CUT&RUN, CUT&Tag, DB, ICC, IF, WB | 100 µg | 39915 | $510 | Buy |
| 50 µg | 39016 | $295 | Buy | ||||
| 10 µg | 39916 | $125 | Buy | ||||
| Histone H3K9ac antibody (pAb) | IgG | Human, Mouse, Wide Range Predicted | ChIP, ChIP-Seq, CUT&Tag, DB, ICC, IF, WB | 100 µg | 39917 | $510 | Buy |
| 50 µg | 39017 | $295 | Buy | ||||
| 10 µg | 39918 | $125 | Buy |
