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Human Control qPCR Primer Sets

Human Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with human samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple human cell lines.

Click the Human qPCR Primer Sets tab below for information on the target of each primer set and what it is an appropriate control for.

 
Name Format Cat No. Price  
Human Negative Control Primer Set 1 96 rxns 71001 $90 Buy Now
Human Negative Control Primer Set 2 96 rxns 71002 $90 Buy Now
Human Negative Control Primer Set 3 96 rxns 71023 $90 Buy Now
Human Positive Control Primer Set ACTB-1 96 rxns 71003 $90 Buy Now
Human Positive Control Primer Set ACTB-2 96 rxns 71005 $90 Buy Now
Human Positive Control Primer Set CCND2 96 rxns 71008 $90 Buy Now
Human Positive Control Primer Set GAPDH-1 96 rxns 71004 $90 Buy Now
Human Positive Control Primer Set GAPDH-2 96 rxns 71006 $90 Buy Now
Human Positive Control Primer Set GEMIN4 96 rxns 71010 $90 Buy Now
Human Positive Control Primer Set MYT1 96 rxns 71007 $90 Buy Now
Human Positive Control Primer Set ZC3H13 96 rxns 71009 $90 Buy Now

Human Chromatin IP Control qPCR Primer Sets

Cat. No.   Name Gene Control for...
71001 Human Negative Control Primer Set 1 Gene desert on chromosome 12 Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71002 Human Negative Control Primer Set 2 Gene desert on chromosome 4 Any transcription factor, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71023 Human Negative Control Primer Set 3 ACTB promoter H3K27me3, EZH2
71003 Human Positive Control Primer Set ACTB-1 ACTB intron 3 RNA pol II phospho Ser2, H3K36me3
71004 Human Positive Control Primer Set GAPDH-1 GAPDH intron 7 RNA pol II phospho Ser2, H3K36me3
71005 Human Positive Control Primer Set ACTB-2 ACTB promoter H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, HDAC1, LSD1, Total RNA pol II, RNA pol II phospho Ser5
71006 Human Positive Control Primer Set GAPDH-2 GAPDH intron 1 H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, Total RNA pol II, RNA pol II phospho Ser5
71007 Human Positive Control Primer Set MYT1 MYT1 promoter H3K27me3, EZH2
71008 Human Positive Control Primer Set CCND2 CCND2 promoter H3K27me3, EZH2, Suz12
71009 Human Positive Control Primer Set ZC3H13 ZC3H13 exon 8 5-mC (MeDIP)
71010 Human Positive Control Primer Set GEMIN4 Region 3´ of GEMIN4 5-mC (MeDIP)

 

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.