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HDAC Assay Kits are 96-well plate-based assays to determine the HDAC (Histone Deacetylase) activity in your extracts or purified samples. HDAC Assay Kits are available in both colorimetric and fluorescent formats to suit your needs.
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| HDAC Assay Kit (Fluorescent) | 1 x 96 rxns | 56200 | $375 | Buy Now |
| HDAC Assay Kit (Colorimetric) | 1 x 96 rxns | 56210 | $375 | Buy Now |
Each HDAC Assay Kit contains positive control HeLa nuclear extract, deacetylated HDAC assay standard, HDAC peptide substrate, Trichostatin A as a model inhibitor and all the essential buffers for a full 96-well plate assay.
Assay advantages
- Works with Class I, IIB and IV HDAC enzymes (does not work with HDAC Class IIA)
- Easy to adapt to work with Class III HDAC enzymes
- Use nuclear extracts, immunoprecipitates, column fractions or purified proteins
- Calculate enzymatic activity and screen inhibitor compounds
Figure 1: Colorimetric HDAC Assay results.
HeLa nuclear extracts were assayed from 0 to 50 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.
Figure 2: Fluorescent HDAC Assay results.
HeLa nuclear extracts were assayed from 0 to 10 µg per well in duplicate. The purple line represents activity from untreated extracts, while the copper line represents extracts treated with 1 µM Trichostatin A inhibitor.
Contents & Storage
HDAC Assay Buffer, HDAC Substrate, HDAC Assay Standard, Trichostatin A, HDAC Developer Solution, HeLa Nuclear Extract, 96-well half-volume plate. HeLa Nuclear Extract is to be stored at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.
