HistonePath ChIP-Seq provides accurate, reproducible identification of histone modifications across the genome with high sensitivity and specificity. This technique is widely used in stem cell research and in understanding disease progression or response to cellular treatment. It can also be useful in identifying new biomarkers since histone modification patterns can be predicitve of gene expression and thus be detected prior to changes in gene expression.
Figure 1: ChIP-Seq of small molecular inhibitor treated cells.
ChIP-Seq was performed from CD34+ cells and antibodies against a histone acetyltransferase (HAT) and its corresponding histone modification. In the presence of a small molecule inhibitor the occupancy of the histone acetyltransferase was specifically reduced at the promoter of the RBBP4 gene but not at the other genes within the 150,000 bp window shown. As expected, the corresponding histone tail acetylation was also reduced at the RBBP4 gene.
Figure 2: HistonePath™ ChIP-Seq reveals that H3K4me3 peaks are present at the start site of all Zfp genes.
ChIP-Seq was performed using chromatin from mouse livers and a Histone H3 trimethyl Lys4 polyclonal antibody (Catalog No. 39159). Sequencing was performed on the Illumina GA II using 36 bp single-end reads. Genome alignment was performed with ELAND and peak calling was performed with MACS. The image above focuses on a 3 Mb window containing a Zfp gene cluster on chromosome 13. H3K4me3 peaks are present at the start site of all Zfp genes. Gene annotations run from right to left, therefore the transcription start sites are on the right side of each gene annotation.