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Our Epigenetic Services team specializes in performing ChIP-Seq and ChIP-chip. Because of the need for high-quality antibodies in order to achieve success in these techniques, we validate all antibodies internally before using them in genome-wide ChIP assays. To date, we have ChIP-qualified antibodies against more than 200 different targets. If your target of interest is on our list, we can start your project immediately. If it is not, or if you want to ChIP validate your own internally developed antibody, our Antibody Validation Service can give you an answer in as little as two weeks. Active Motif Epigenetic Services’ offers antibody validation by ChIP-Seq and by ChIP-chip. Please contact us to find out which method is the most appropriate for your project.
To learn more, please give us a call or send us an Epigenetic Services Information Request. You can also download Active Motif’s Epigenetic Services Profile.
ChIP Antibody Validations Services.
Active Motif performs all steps of the ChIP experiment from chromatin preparation to data analysis, and provides an evaluation of the antibody’s performance.
| Name | Cat No. | Price | |
|---|---|---|---|
| Antibody Qualification (ChIP-Seq) | 25054 | Request Quote | |
| Antibody Qualification (ChIP-chip) | 25043 | Request Quote | |
| Active Motif Epigenetic Services Profile |
| Epigenetic Services Cell Fixation Protocol |
| Epigenetic Services Sample Submission Form |
Antibody validation by ChIP-Seq
ChIP-Seq antibody validation is a small scale, ChIP-Seq reaction.
- More robust and more sensitive than ChIP-chip
- Sequencing performed on an Illumina MiSeq
- 3-4 week turnaround
- Recommended when the antibody target is expected to be challenging
Antibody validation by ChIP-chip
ChIP-chip antibody validation is based on hybridization to an array.
- Less expensive than ChIP-Seq
- Hybridization to a single Affymetrix tiling array
- 2-3 week turnaround
- Recommended when antibody performance is expected to be robust
Use of ChIP-chip for antibody validation.
The ChIP Antibody Validation Service by ChIP-chip includes sourcing a candidate antibody, performing ChIP using chromatin from the customer’s formaldehyde-fixed cells (or an appropriate internal cell line), amplification, fragmentation and labeling of the ChIP DNA, and hybridization to an Affymetrix tiling array. The data is then analyzed and it is determined if the antibody is suitable for ChIP based on the number, intensity and shape of the peaks identified on the array. If the antibody is found to be acceptable, it can be used for ChIP as well as for genome-wide ChIP-Seq and ChIP-chip assays.
