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Fully Methylated Jurkat DNA

CpG-methylated genomic DNA for methylation experiments

Active Motif offers Fully Methylated Jurkat DNA for use as a control in your DNA methylation applications. Whether you're performing bisulfite sequencing, methylation-specific PCR (MSP), MeDIP or using our MethylCollector™ or MethylDetector™ Kits, Fully Methylated Jurkat DNA is a convenient positive control for investigating CpG dinucleotide methylation.

Fully Methylated Jurkat DNA is supplied with a BRCA1 primer set. As native Jurkat DNA is not methylated at the BRCA1 locus, this primer set is ideal for use as a control in methylation-specific experiments with Fully Methylated Jurkat DNA.

 
Name Format Cat No. Price  
Fully Methylated Jurkat DNA 10 µg 55003 $250 Buy Now
Jurkat genomic DNA 10 µg 55007 $60 Buy Now
 
 
Figure 1: Fully Methylated Jurkat DNA used as a control for MethylCollector™.

The MethylCollector Kit was used to assay 100 ng genomic DNA (lanes 2 & 4) and Fully Methylated Jurkat DNA (lanes 3 & 5) samples with (lanes 4 & 5) or without (lanes 2 & 3) inclusion of 1 µg of the kit’s His-tagged MBD2b protein. These samples, the input genomic DNA (lane 6) and the input Fully Methylated Jurkat DNA (lane 7) were then PCR amplified with the BRCA1 primers. Lane 8 is a water-only PCR control. A positive result is observed only with Fully Methylated Jurkat DNA captured by MethylCollector’s His-tagged MBD2b (lane 5), demonstrating that MethylCollector specifically isolates methylated DNA and that Fully Methylated Jurkat DNA is a useful control in methylation-specific experiments.

Contents & Storage

10 µg of Fully Methylated Jurkat DNA (200 µl at 50 ng/µl), and BRCA1 PCR Primer Mix. Store at -20°C. All reagents guaranteed for 6 months when stored properly.

Jurkat genomic DNA contains 10 µg (50 ng/µl) undigested genomic DNA.