Bridging Antibody for Mouse IgG
increased affinity of mouse primary antibodies in immunoprecipitation
Some isotypes of mouse antibody do not bind well to the protein G-conjugated agarose/magnetic beads used in standard immunoprecipitation and chromatin immunoprecipitation experiments. This can be improved by the inclusion of an anti-mouse IgG bridging antibody, which binds with a strong affinity to both the protein G beads and the mouse primary antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, use of the Bridging Antibody for Mouse IgG helps improve the results of all ChIP and IP experiments that use mouse primary antibody.
|Bridging Antibody for Mouse IgG Data Sheet|
|Active Motif Antibodies Brochure|
|Chromatin & Epigenetics Products Brochure|
|MSDS: Sodium Azide|
The Bridging Antibody for Mouse IgG helps improve immunoprecipitation (ChIP and IP) experiments that use primary mouse antibody by improving the affinity of the mouse primary antibody for the protein G-conjugated agarose/magnetic beads that are used in immunoprecipitation (Figure 1).
Figure 1: Improvement in ChIP using the Bridging Antibody for Mouse IgG.
Contents & Storage
Five hundred µg of affinity-purified anti-mouse IgG in PBS, 30% glycerol and 0.035% sodium azide. Store at -20°C. Guaranteed stable for 6 months when stored properly.