Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

STAY INFORMED

Sign up to receive new product updates
and promotional pricing.

Bridging Antibody for Mouse IgG

increased affinity of mouse primary antibodies in immunoprecipitation

Some isotypes of mouse antibody do not bind well to the protein G-conjugated agarose/magnetic beads used in standard immunoprecipitation and chromatin immunoprecipitation experiments. This can be improved by the inclusion of an anti-mouse IgG bridging antibody, which binds with a strong affinity to both the protein G beads and the mouse primary antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, use of the Bridging Antibody for Mouse IgG helps improve the results of all ChIP and IP experiments that use mouse primary antibody.

 
Name Format Cat No. Price  
Bridging Antibody for Mouse IgG 500 µg 53017 $90 Buy Now

The Bridging Antibody for Mouse IgG helps improve immunoprecipitation (ChIP and IP) experiments that use primary mouse antibody by improving the affinity of the mouse primary antibody for the protein G-conjugated agarose/magnetic beads that are used in immunoprecipitation (Figure 1).

 
 
Figure 1: Improvement in ChIP using the Bridging Antibody for Mouse IgG.

ChIP was performed using chromatin from U-937 cells induced with TNF-α (10 ng/ml for one hour). PCR was performed with primers corresponding to the human IL-8 promoter.

Lanes 1-4: Beads pre-incubated with no bridging antibody.
Lanes 5-8: Beads pre-incubated with 5 µg bridging antibody.
Lanes 1, 2, 5 & 6: ChIP performed using NFκB p65 mouse monoclonal antibody, 2 µg per IP.
Lanes 3, 4, 7 & 8: ChIP performed using negative control mouse IgG.

Contents & Storage

Five hundred µg of affinity-purified anti-mouse IgG in PBS, 30% glycerol and 0.035% sodium azide. Store at -20°C. Guaranteed stable for 6 months when stored properly.