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| Western Blot | Chromatin IP | Immunoprecipitation |
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| 2 - 4 µg/ml | 3rd-party validated* | 3rd-party validated* |
| *This antibody has been reported to work in this application by a third party, but Active Motif has not tested the antibody for this application. Please see “Application Notes” below for any recommendations. | ||
| View Technical Data Sheet (TDS) |
Background for DNMT1 antibody (mAb):
DNMT1 – DNA methyltransferase 1 (or DNMT 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.
Chromatin IP Assays:
Active Motif has Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page
Immunogen for DNMT1 antibody (mAb):
This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.Application Notes for DNMT1 antibody (mAb):
This DNMT1 antibody has been validated for use in Western blotting (2 - 4 µg/ml) and has been reported to work in chromatin IP, immunoprecipitation and immunohistochemistry. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.Buffer:
PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.Positive Control:
Whole-cell extracts from mouse ES cells can be used as a positive control for DNMT1 antibody.Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
References:
This antibody was used in ChIP and in Re-ChIP assays as described in Schnekenburger, M. et. al. (2007) Mol Cell Biol. 27(20): 7089–7101.
DNMT1 antibody (mAb) tested by Western blot.
Detection of DNMT1 by Western blot. The analysis was performed using mouse ES whole-cell extract and DNMT1 antibody (mAb) at a 2 µg/ml dilution.
Storage:
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
