| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG |
| Purification: | Protein G Chromatography |
|
| Appl.: | WB |
| Reactivity: | Human, Mouse |
| Molecular Weight: | 100 kDa |
|
| Western Blot |
Chromatin IP |
| 0.5 - 2.0 µg/ml |
Not tested |
Background for Rad21 antibody (mAb) (Clone 52A311):
Rad21 Clone 52A311 (
HR21,
MCD1,
NXP1,
hHR21) is highly similar to the gene product of
S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis.
Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in
cell cycle M phase. The highly regulated association of
Rad21 with mitotic
chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Immunogen for Rad21 antibody (mAb) (Clone 52A311):
Recombinant protein corresponding to the N-terminal half of mouse Rad21.
Application Notes for Rad21 antibody (mAb) (Clone 52A311):
This Rad21 antibody (Clone 52A311) has been validated for use in Western blotting (0.5 - 2 µg/ml dilution).
Buffer:
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
Positive Control:
Active Motif's
HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Rad21 antibody (Clone 52A311).
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Rad21 mAb (Clone 52A311) tested by Western blot.
HeLa nuclear extract (30 µg per lane) was probed with Rad21 mAb (Clone 52A311) (1 µg/ml).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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