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PARP-1 C-terminal antibody (pAb)
| Catalog No: 39561 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39562 | Format: 10 µl | $70 | Buy Now |
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| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1:1,000 - 1:6,000 | 10 µl per ChIP | Published. See application notes. |
for PARP-1 C-terminal antibody (pAb)Background
PARP-1 C-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.
For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.
for PARP-1 C-terminal antibody (pAb)Immunogen
His-tagged fusion protein corresponding to the C-terminal half of human PARP-1 was used to generate this PARP-1 antibody.
for PARP-1 C-terminal antibody (pAb)Application Notes
This PARP-1 C-terminal antibody has been validated for use in Western blotting (1:1,000 - 1:6,000 dilution), chromatin IP (ChIP, 10 µl per ChIP) and reported to work in immunohistochemistry and immunoprecipitation. See references for additional information.
for PARP-1 C-terminal antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for PARP-1 C-terminal antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PARP-1 C-terminal antibody.
Chromatin IP Kits & Reagents
Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with PARP-1 C-terminal antibody (pAb).
for PARP-1 C-terminal antibody (pAb)References
This antibody has been used in the following references:
Kim MY et al (2004) Cell 119: 803-814.
Krishnakumar R et al (2008) Science 319: 819-821.
PARP-1 C-terminal antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.
PARP-1 C-terminal antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF7 chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of PARP-1 C-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for PARP-1 C-terminal antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PARP-1 C-terminal antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
