YOUR CART
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| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:1,000 - 1:2,500 | Not tested | 1:250 - 1:1,000 |
| View Technical Data Sheet (TDS) |
Background for Histone macroH2A1 antibody (pAb):
Histone macroH2A (mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated. In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription.MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD).
Immunogen for Histone macroH2A1 antibody (pAb):
This antibody was raised against recombinant protein corresponding to the N-terminal half of mouse macroH2A1.2, so will react with macroH2A1 and the macroH2A1.1 and macroH2A1.2 isoforms.Application Notes for Histone macroH2A1 antibody (pAb):
This Histone macroH2A1 antibody has been validated for use in Western blotting (1:1,000 - 1:2,500 dilution) and immunofluorescence (1:250 - 1:1,000 dilution).Buffer:
Rabbit serum containing 30% glycerol, 0.035% sodium azide.Positive Control:
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone macroH2A1 antibody.Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
Histone macroH2A1 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000.
Histone macroH2A1 pAb tested by immunofluorescence.
Staining of HeLa cells with Histone macroH2A1 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
