Bub1 antibody (mAb)
RRID: AB_2793325 |
Clone: 14H5 |
Aliases: BUB1A, BUB1L, hBUB1 |
Catalog No: 39735 | Format: 100 µg | $320 | Buy |
Catalog No: 39736 | Format: 10 µg | $105 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
ICC/IF
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Bub1 antibody was raised against a recombinant protein corresponding to amino acids 281-419 of human Bub1.
Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Background
Bub1 is a serine-threonine protein kinase and an important regulator of cell division. It is essential for the G2/M mitotic spindle assembly/integrity checkpoint as well as critical for chromosome alignment. Bub1 is required for the assembly of a large number of proteins at the kinetochore, including CENP-E, CENP-F, BUB1B, MAD2L1 and PLK1. It plays a role in sister chromatid cohesion and it serves as a substrate for the anaphase-promoting complex. Bub1 mediates cell death in response to chromosome mis-segregation and acts to suppress spontaneous tumorigenesis.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting