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Antibodies

Histone H3K9ac antibody (pAb)

Catalog No: 39917 Format: 100 µg $420 Buy Now
Catalog No: 39918 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 acetyl Lys9 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
ChIP-Seq: 3 µg each
ICC/IF: 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here..

Published Applications:
ChIP
WB
See references for more information. Individual optimization may be required.

for Histone H3 acetyl Lys9 antibody (pAb)Immunogen

This Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of histone H3.

for Histone H3 acetyl Lys9 antibody (pAb)Buffer

Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39137) of this antibody is also available.

for Histone H3 acetyl Lys9 antibody (pAb)References

 
Histone H3K9ac antibody (pAb) tested by ChIP-Seq.

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver. ChIP DNA was sequenced on the Illumina GA II and 25 million sequence tags were mapped to identify H3K9Ac binding across the genome. The image shows a 1.5 million base pair region on chromosome 15. H3K9Ac shows promoter localization at many genes and broader binding near the Gcat gene.

Histone H3K9ac antibody (pAb) tested by ChIP.

ChIP of Histone H3 acetyl Lys9 pAb.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H3 acetyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3K9ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.
HeLa cells stained at 2 µg/ml with Histone H3 acetyl Lys9 antibody. Top panel: Histone H3 acetyl Lys9 antibody. Middle panel: DAPI. Bottom panel: merge.

Histone H3K9ac antibody (pAb) tested by Western blot.

Western blot of Histone H3 acetyl Lys9 antibody (pAb).
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys9 antibody (1 µg per ml).
Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.

Histone H3K9ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys9 antibody for acetyl Lys9 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 acetyl Lys9 antibody at a dilution of 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: histone H3 acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys9/14/18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl- Lys14 peptide. Lane 7: acetyl-Lys18 peptide. Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide. Lane 10: unmodified Lys27 peptide.

for Histone H3 acetyl Lys9 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions.

Histone H3 Lys9 can also be mono-, di- or trimethylated. The methylation of this residue is often associated with transcriptional repression. However, acetylation of histone H3 Lys9 is associated with transcriptional activation of the genes.

for Histone H3 acetyl Lys9 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 acetyl Lys9 antibody.

for Histone H3 acetyl Lys9 antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K9ac antibody (pAb).

for Histone H3 acetyl Lys9 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys9 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K9ac antibody (pAb)
 

Data Thumbnails

Histone H3K9ac antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H3K9ac pAb.
(Click image to enlarge and see details.)

Histone H3K9ac antibody (pAb) tested by ChIP.

ChIP of Histone H3K9ac pAb.
(Click image to enlarge and see details.)

Histone H3K9ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3K9ac pAb.
(Click image to enlarge and see details.)

Histone H3K9ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K9ac pAb.
(Click image to enlarge and see details.)

Histone H3K9ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K9ac pAb.
(Click image to enlarge and see details.)