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Histone H4 dimethyl Arg3, symmetric antibody (pAb)
| Catalog No: 61187 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61188 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.
Symmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional repression. Histone H4 dimethyl Arg3 symmetric methylation (H4R2me2s) is catalyzed by PRMT5 and PRMT7.
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Immunogen
This Histone H4 dimethyl Arg3 (H3R3me2s) antibody was raised against a peptide containing dimethyl Arg3 (symmetric) of human Histone H4.
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Application Notes
This Histone H4 dimethyl Arg3 symmetric (H3R3me2s) antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 dimethyl Arg3, asymmetric antibody.
Histone H4 dimethyl Arg3 symmetric pAb tested by Western blot.
Western blot performed using Histone H4 dimethyl Arg3 symmetric pAb at a dilution of 1:2,000. Lane 1: Nuclear extract from Raji cells (20 µg). Lane 2: Nuclear extract from HeLa cells (20 µg).
Histone H4 dimethyl Arg3 symmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 symmetric pAb for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 symmetric pAb at 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified Arg3. Lane 2: dimethyl-Arg3 H4 (symmetric). Lane 3: dimethyl-Arg3 H4 (asymmetric). Lane 4: monomethyl-Arg3 H4. Lane 5: unmodified Arg26 H3. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asymmetric). Lane 8: dimethyl-Arg26 H3 (symmetric). Lane 9: dimethyl-Arg2 H3 (asymmetric). Lane 10: dimethyl-Arg2 (symmetric). Lane 11: dimethyl-Arg8 H3 (asymmetric). Lane 12: dimethyl-Arg8 H3 (symmetric). Lane 13: dimethyl-Arg17 (asymmetric). Lane 14: dimethyl-Arg17 H3 (symmetric).
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 dimethyl Arg3, symmetric antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
