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Antibodies

MCM2 antibody (pAb)

Aliases: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN
Catalog No: 61717 Format: 100 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:115 kDa
Reactivity:Human

Applications

Western Blot Validated

for MCM2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for MCM2 antibody (pAb)Immunogen

This antibody was raised against a full-length recombinant protein corresponding to human MCM2.

for MCM2 antibody (pAb)Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

 
MCM2 antibody (pAb) tested by Western blot.

MCM2 antibody (pAb) tested by Western blot.
MCM2 detection by Western blot. The analysis was performed using MCF7 nuclear cell extract (20 µg) and MCM2 antibody at a 1:1,000 dilution.

for MCM2 antibody (pAb)Background

MCM2 (Minichromosome Maintenance Complex Component 2) acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

for MCM2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MCM2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MCM2 antibody (pAb)
 

Data Thumbnails

MCM2 antibody (pAb) tested by Western blot.

Western blot of MCM2 pAb.
(Click image to enlarge and see details.)